Cytochalasin b glucose uptake

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WebMar 5, 2010 · When expressed in S. cerevisae, rat GLUT1 showed a Km for D -glucose of 3.4 mM and transport was inhibited by cytochalasin B (IC50 = 0.44 μM), HgCl 2 (IC50 = 3.5 μM), phloretin (IC50 = 49 μM), and … Web2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin …

Cytochalasin B - an overview ScienceDirect Topics

WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the WebIn erythrocytes, adipocytes, and chick embryo fibroblasts, the Ki for glucose uptake inhibition and the Kd for cytochalasin B binding range from 0.1 to 0.4 µM. Values for hepatocytes and liver membranes are an order of magnitude higher … five go on a hike together

The inhibition of glucose uptake to erythrocytes: microwave

WebCytochalasin B has been shown to potently inhibit the transport of glucose, deoxyglucose, and glucosamine by Novikoff hepatoma cells in suspension culture without affecting their … WebThe widely expressed mammalian glucose transporter, GLUT1, can be acutely activated in L929 fibroblast cells by a variety of conditions, including glucose deprivation, or treatment with various respiration inhibitors.Known thiol reactive compounds including phenylarsine oxide and nitroxyl are the fastest acting stimulators of glucose uptake, implicating … WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … five go sailing youtube

Glucose Transport and Glucose Transporters in Muscle and Their ...

Discovery of a specific inhibitor of human GLUT5 by …

WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … WebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. can iphone 5c still be usedWebNov 11, 2008 · Non-carrier-mediated uptake was determined in parallel wells containing 10 μmol/L cytochalasin B. Protein concentration was measured by the modified Bradford protein assay. Cell-associated radioactivity was determined by scintillation counting. The mean counts/min were expressed as pmol/min/mg protein from determinations done in … can iphone 5 play imessage games

"WebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

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WebEnter the email address you signed up with and we'll email you a reset link. WebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures .

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WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. WebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular

WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. … Web(B) Glucose uptake was assayed by incubation of control, cytochalasin B-, and cytochalasin D-treated Jurkat cells in the presence of 2-deoxy-D [1-3H]glucose (5 μM) …

WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … WebMar 1, 1990 · Glucose uptake in muscle occurs by a system of facilitated diffusion involving at least two distinct glucose transporters, GLUT-1 and GLUT-4. Both bind the fungal …

WebJan 1, 1990 · Cytochalasin B also blocked the uptake of 14 C-L-ascorbic acid (1.25 mg%). The results of these studies further support the hypothesis that glucose and ascorbate …

WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... five gospels in parallelWebIn vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter. Chih Hsiung Wu, Yuan Soon Ho, Chia Yi Tsai, Ying Jan Wang, How Tseng, Po Li Wei, Chia Hwa Lee, Ren Shyan Liu, Shyr Yi Lin. five go parenting bookCytochalasin B inhibits glucose transport [3] and platelet aggregation. It blocks adenosine -induced apoptotic body formation without affecting activation of endogenous ADP -ribosylation in leukemia HL-60 cells. [4] It is also used in cloning through nuclear transfer. See more Cytochalasin B, the name of which comes from the Greek cytos (cell) and chalasis (relaxation), is a cell-permeable mycotoxin. It was found that substoichiometric concentrations of cytochalasin B (CB) strongly inhibit … See more 1960s Cytochalasin B was first described in 1967, when it had been isolated from moulds by Dr W.B. Turner. Smith et al. found that CB … See more Cytochalasin B contains several highly polar keto- and hydroxyl groups and one peripheric lipophilic benzyl unit. See more There are ten possible sites for the in vitro degradation of cytochalasin B. There is not yet any evidence that the same sites are used for degradation in vivo, but evidence has confirmed the in vitro sites. Degradation is initialized by a periodate cleavage of the … See more Cytochalasins can be isolated from the fungi in which they naturally occur. Originally, they were isolated from Helminthosporium dematioideum. Other producers include See more It is suggested that the predominant mechanism of cytochalasin B is the inhibition of actin filament polymerization through binding to … See more Interactions When adding cytochalasin B and the beta-andrenergic agonist (-)-isoproterenol, prostaglandin E1 or cholera toxin to wild type S49 … See more can iphone 5 still be usedWebApr 14, 2016 · Glucose uptake for GlcP Se was measured one minute after initiation of transport with 30 μM C 14 -glucose in the presence of 2 mM … can iphone 5s get ios 15WebMay 13, 2024 · A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo. ... To examine the effect of cytochalasin B ... can iphone 6 be traded inWebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... can iphone 5s use 4g network five gothic conventions